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Fig. 5 | SpringerPlus

Fig. 5

From: Rapid sample processing for intracellular metabolite studies in Penicillium ochrochloron CBS 123.824: the FiltRes-device combines cold filtration of methanol quenched biomass with resuspension in extraction solution

Fig. 5

Intracellular nucleotide concentrations applying the FiltRes-principle on Penicillium ochrochloron CBS 123.824 grown under glucose-limited chemostat conditions at µ = 0.1 h−1. a Comparison of intracellular metabolite concentrations derived from samples with identical sample extraction protocol and analytical procedure (steady states from three independent chemostat cultivations; light grey bars chemostat III, dark grey bars chemostat IV, white bars chemostat VI at 42, 73 and 88 h). Hatched bars indicate data derived from heat stopped total broth samples (controls). Data are averages of at least three samples. Details to each chemostat see Table 1. Inosine and IMP were not detected. b Effect of washing steps (WS) with −40 °C cold 60 % (v/v) methanol on the intracellular metabolite concentrations. Inosine and IMP were not detected. Data were obtained from chemostat III (see Table 1) and are averages of three samples with exception of the samples with two washing steps, which were performed in duplicates

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