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Table 5 The stability of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte Nominal concentration (ng/mL) Short-term Freeze–thaw cycles Long-term
25 °C for 4 h (RSD%) Three freeze–thaw cycles (RSD%) Frozen for 2 weeks (RSD%)
Astragaloside IV 74 0.35 1.21 2.05
740 0.47 1.17 0.89
7400 0.96 0.74 1.15
Cycloastragenol 7.4 0.59 2.1 0.78
74 1.47 0.99 1.04
740 0.85 4.07 1.54
Calycosin-7-O-β-d-glucoside 74 0.76 1.59 2.19
740 0.97 2.75 0.68
7400 2.41 1.34 1.3
Calycosin 7.4 1.02 3.52 2.11
74 0.9 1.57 2.54
740 1.53 3.79 1.15
Ononin 74 1.2 1.45 0.93
740 0.92 2.97 1.06
7400 2.36 3.11 2.35
Formononetin 7.4 1.14 0.82 1.47
74 0.88 1.09 0.38
740 0.56 1.18 1.02