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Table 5 The stability of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte

Nominal concentration (ng/mL)

Short-term

Freeze–thaw cycles

Long-term

25 °C for 4 h (RSD%)

Three freeze–thaw cycles (RSD%)

Frozen for 2 weeks (RSD%)

Astragaloside IV

74

0.35

1.21

2.05

740

0.47

1.17

0.89

7400

0.96

0.74

1.15

Cycloastragenol

7.4

0.59

2.1

0.78

74

1.47

0.99

1.04

740

0.85

4.07

1.54

Calycosin-7-O-β-d-glucoside

74

0.76

1.59

2.19

740

0.97

2.75

0.68

7400

2.41

1.34

1.3

Calycosin

7.4

1.02

3.52

2.11

74

0.9

1.57

2.54

740

1.53

3.79

1.15

Ononin

74

1.2

1.45

0.93

740

0.92

2.97

1.06

7400

2.36

3.11

2.35

Formononetin

7.4

1.14

0.82

1.47

74

0.88

1.09

0.38

740

0.56

1.18

1.02

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