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Table 4 Mean extraction recoveries and matrix effects of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte

Spiked concentration (ng/mL)

Recovery (%)

Matrix effect (%)

Mean ± SD

RSD

Mean ± SD

RSD

Astragaloside IV

74

99.64 ± 0.002

0.28

99.81 ± 0.004

0.49

740

99.61 ± 0.004

0.49

99.65 ± 0.012

1.27

7400

96.46 ± 0.066

6.91

99.71 ± 0.024

2.41

Cycloastragenol

7.4

100.54 ± 0.031

3.17

102.70 ± 0.09

8.80

74

98.21 ± 0.058

6.00

100.74 ± 0.063

6.25

740

100.13 ± 0.004

0.49

99.96 ± 0.011

1.18

Calycosin-7-O-β-d-glucoside

74

99.20 ± 0.016

1.68

97.71 ± 0.05

5.20

740

99.21 ± 0.01

1.10

98.53 ± 0.022

2.27

7400

99.33 ± 0.015

1.54

101.50 ± 0.028

2.76

Calycosin

7.4

98.63 ± 0.026

2.71

98.89 ± 0.018

1.88

74

97.17 ± 0.063

6.50

98.48 ± 0.042

4.31

740

93.63 ± 0.081

8.73

95.33 ± 0.111

11.64

Ononin

74

99.57 ± 0.006

0.69

100.69 ± 0.013

1.36

740

98.95 ± 0.017

1.77

101.47 ± 0.024

2.46

7400

98.64 ± 0.021

2.17

97.47 ± 0.052

5.37

Formononetin

7.4

95.49 ± 0.07

7.36

99.65 ± 0.059

5.97

74

97.55 ± 0.039

4.01

100.44 ± 0.007

0.78

740

95.39 ± 0.081

8.59

104.36 ± 0.068

6.57