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Table 4 Mean extraction recoveries and matrix effects of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte Spiked concentration (ng/mL) Recovery (%) Matrix effect (%)
Mean ± SD RSD Mean ± SD RSD
Astragaloside IV 74 99.64 ± 0.002 0.28 99.81 ± 0.004 0.49
740 99.61 ± 0.004 0.49 99.65 ± 0.012 1.27
7400 96.46 ± 0.066 6.91 99.71 ± 0.024 2.41
Cycloastragenol 7.4 100.54 ± 0.031 3.17 102.70 ± 0.09 8.80
74 98.21 ± 0.058 6.00 100.74 ± 0.063 6.25
740 100.13 ± 0.004 0.49 99.96 ± 0.011 1.18
Calycosin-7-O-β-d-glucoside 74 99.20 ± 0.016 1.68 97.71 ± 0.05 5.20
740 99.21 ± 0.01 1.10 98.53 ± 0.022 2.27
7400 99.33 ± 0.015 1.54 101.50 ± 0.028 2.76
Calycosin 7.4 98.63 ± 0.026 2.71 98.89 ± 0.018 1.88
74 97.17 ± 0.063 6.50 98.48 ± 0.042 4.31
740 93.63 ± 0.081 8.73 95.33 ± 0.111 11.64
Ononin 74 99.57 ± 0.006 0.69 100.69 ± 0.013 1.36
740 98.95 ± 0.017 1.77 101.47 ± 0.024 2.46
7400 98.64 ± 0.021 2.17 97.47 ± 0.052 5.37
Formononetin 7.4 95.49 ± 0.07 7.36 99.65 ± 0.059 5.97
74 97.55 ± 0.039 4.01 100.44 ± 0.007 0.78
740 95.39 ± 0.081 8.59 104.36 ± 0.068 6.57