Analyte | Spiked concentration (ng/mL) | Recovery (%) | Matrix effect (%) | ||
---|---|---|---|---|---|
Mean ± SD | RSD | Mean ± SD | RSD | ||
Astragaloside IV | 74 | 99.64 ± 0.002 | 0.28 | 99.81 ± 0.004 | 0.49 |
740 | 99.61 ± 0.004 | 0.49 | 99.65 ± 0.012 | 1.27 | |
7400 | 96.46 ± 0.066 | 6.91 | 99.71 ± 0.024 | 2.41 | |
Cycloastragenol | 7.4 | 100.54 ± 0.031 | 3.17 | 102.70 ± 0.09 | 8.80 |
74 | 98.21 ± 0.058 | 6.00 | 100.74 ± 0.063 | 6.25 | |
740 | 100.13 ± 0.004 | 0.49 | 99.96 ± 0.011 | 1.18 | |
Calycosin-7-O-β-d-glucoside | 74 | 99.20 ± 0.016 | 1.68 | 97.71 ± 0.05 | 5.20 |
740 | 99.21 ± 0.01 | 1.10 | 98.53 ± 0.022 | 2.27 | |
7400 | 99.33 ± 0.015 | 1.54 | 101.50 ± 0.028 | 2.76 | |
Calycosin | 7.4 | 98.63 ± 0.026 | 2.71 | 98.89 ± 0.018 | 1.88 |
74 | 97.17 ± 0.063 | 6.50 | 98.48 ± 0.042 | 4.31 | |
740 | 93.63 ± 0.081 | 8.73 | 95.33 ± 0.111 | 11.64 | |
Ononin | 74 | 99.57 ± 0.006 | 0.69 | 100.69 ± 0.013 | 1.36 |
740 | 98.95 ± 0.017 | 1.77 | 101.47 ± 0.024 | 2.46 | |
7400 | 98.64 ± 0.021 | 2.17 | 97.47 ± 0.052 | 5.37 | |
Formononetin | 7.4 | 95.49 ± 0.07 | 7.36 | 99.65 ± 0.059 | 5.97 |
74 | 97.55 ± 0.039 | 4.01 | 100.44 ± 0.007 | 0.78 | |
740 | 95.39 ± 0.081 | 8.59 | 104.36 ± 0.068 | 6.57 |