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Table 3 The intra-day and inter-day accuracies and precisions of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte Nominal concentration (ng/mL) Intra-day(n = 6) Inter-day (n = 6)
Observed concentration (ng/mL) Precision (RSD%) Accuracy (RE%) Observed concentration (ng/mL) Precision (RSD%) Accuracy (RE%)
Astragaloside IV 74 73.1 ± 0.11 1.46 −1.22 73.8 ± 0.14 3.25 −0.25
740 737 ± 1.23 1.64 −0.47 746 ± 1.73 2.28 0.79
7400 7395 ± 13.2 1.78 0.34 7505 ± 31.2 4.16 4.24
Cycloastragenol 7.4 7.39 ± 0.11 1.43 0.52 7.47 ± 0.21 2.85 4.35
74 73.7 ± 1.21 1.64 −0.47 73.1 ± 1.31 1.72 −1.22
740 742.8 ± 6.63 0.89 0.79 726.3 ± 2.43 3.24 0.88
Calycosin-7-O-β-d-glucoside 74 73.7 ± 1.29 1.64 0.89 73.1 ± 1.3 1.72 −3.18
740 742.8 ± 6.67 0.89 0.79 726.3 ± 2.36 3.24 0.88
7400 7383 ± 14. 7 0.67 −0.89 7393 ± 18.18 2.46 0.59
Calycosin 7.4 7.40 ± 0.17 2.33 −0.02 7.26 ± 0.15 2.01 −1.89
74 74.37 ± 0.79 1.06 0.90 72.63 ± 2.48 3.41 0.88
740 740.83 ± 5.85 0.79 −0.56 738.50 ± 15.15 2.05 0.48
Ononin 74 73.1 ± 0.13 1.72 −0.88 74.4 ± 0.15 2.06 6.87
740 737 ± 1.2 1.64 −0.87 728 ± 1.7 2.40 −1.67
7400 7440 ± 6.7 0.90 0.95 7363 ± 2.01 2.73 2.27
Formononetin 7.4 7.37 ± 0.09 1.27 0.29 7.49 ± 0.21 2.81 4.59
74 73.7 ± 0.12 1.64 −1.14 73.4 ± 1.2 1.64 −0.77
740 741.3 ± 5.6 0.76 0.59 731.3 ± 1.97 2.70 1.57