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Table 3 The intra-day and inter-day accuracies and precisions of the tested analytes (n = 6)

From: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Analyte

Nominal concentration (ng/mL)

Intra-day(n = 6)

Inter-day (n = 6)

Observed concentration (ng/mL)

Precision (RSD%)

Accuracy (RE%)

Observed concentration (ng/mL)

Precision (RSD%)

Accuracy (RE%)

Astragaloside IV

74

73.1 ± 0.11

1.46

−1.22

73.8 ± 0.14

3.25

−0.25

740

737 ± 1.23

1.64

−0.47

746 ± 1.73

2.28

0.79

7400

7395 ± 13.2

1.78

0.34

7505 ± 31.2

4.16

4.24

Cycloastragenol

7.4

7.39 ± 0.11

1.43

0.52

7.47 ± 0.21

2.85

4.35

74

73.7 ± 1.21

1.64

−0.47

73.1 ± 1.31

1.72

−1.22

740

742.8 ± 6.63

0.89

0.79

726.3 ± 2.43

3.24

0.88

Calycosin-7-O-β-d-glucoside

74

73.7 ± 1.29

1.64

0.89

73.1 ± 1.3

1.72

−3.18

740

742.8 ± 6.67

0.89

0.79

726.3 ± 2.36

3.24

0.88

7400

7383 ± 14. 7

0.67

−0.89

7393 ± 18.18

2.46

0.59

Calycosin

7.4

7.40 ± 0.17

2.33

−0.02

7.26 ± 0.15

2.01

−1.89

74

74.37 ± 0.79

1.06

0.90

72.63 ± 2.48

3.41

0.88

740

740.83 ± 5.85

0.79

−0.56

738.50 ± 15.15

2.05

0.48

Ononin

74

73.1 ± 0.13

1.72

−0.88

74.4 ± 0.15

2.06

6.87

740

737 ± 1.2

1.64

−0.87

728 ± 1.7

2.40

−1.67

7400

7440 ± 6.7

0.90

0.95

7363 ± 2.01

2.73

2.27

Formononetin

7.4

7.37 ± 0.09

1.27

0.29

7.49 ± 0.21

2.81

4.59

74

73.7 ± 0.12

1.64

−1.14

73.4 ± 1.2

1.64

−0.77

740

741.3 ± 5.6

0.76

0.59

731.3 ± 1.97

2.70

1.57