Analyte | Nominal concentration (ng/mL) | Intra-day(n = 6) | Inter-day (n = 6) | ||||
---|---|---|---|---|---|---|---|
Observed concentration (ng/mL) | Precision (RSD%) | Accuracy (RE%) | Observed concentration (ng/mL) | Precision (RSD%) | Accuracy (RE%) | ||
Astragaloside IV | 74 | 73.1 ± 0.11 | 1.46 | −1.22 | 73.8 ± 0.14 | 3.25 | −0.25 |
740 | 737 ± 1.23 | 1.64 | −0.47 | 746 ± 1.73 | 2.28 | 0.79 | |
7400 | 7395 ± 13.2 | 1.78 | 0.34 | 7505 ± 31.2 | 4.16 | 4.24 | |
Cycloastragenol | 7.4 | 7.39 ± 0.11 | 1.43 | 0.52 | 7.47 ± 0.21 | 2.85 | 4.35 |
74 | 73.7 ± 1.21 | 1.64 | −0.47 | 73.1 ± 1.31 | 1.72 | −1.22 | |
740 | 742.8 ± 6.63 | 0.89 | 0.79 | 726.3 ± 2.43 | 3.24 | 0.88 | |
Calycosin-7-O-β-d-glucoside | 74 | 73.7 ± 1.29 | 1.64 | 0.89 | 73.1 ± 1.3 | 1.72 | −3.18 |
740 | 742.8 ± 6.67 | 0.89 | 0.79 | 726.3 ± 2.36 | 3.24 | 0.88 | |
7400 | 7383 ± 14. 7 | 0.67 | −0.89 | 7393 ± 18.18 | 2.46 | 0.59 | |
Calycosin | 7.4 | 7.40 ± 0.17 | 2.33 | −0.02 | 7.26 ± 0.15 | 2.01 | −1.89 |
74 | 74.37 ± 0.79 | 1.06 | 0.90 | 72.63 ± 2.48 | 3.41 | 0.88 | |
740 | 740.83 ± 5.85 | 0.79 | −0.56 | 738.50 ± 15.15 | 2.05 | 0.48 | |
Ononin | 74 | 73.1 ± 0.13 | 1.72 | −0.88 | 74.4 ± 0.15 | 2.06 | 6.87 |
740 | 737 ± 1.2 | 1.64 | −0.87 | 728 ± 1.7 | 2.40 | −1.67 | |
7400 | 7440 ± 6.7 | 0.90 | 0.95 | 7363 ± 2.01 | 2.73 | 2.27 | |
Formononetin | 7.4 | 7.37 ± 0.09 | 1.27 | 0.29 | 7.49 ± 0.21 | 2.81 | 4.59 |
74 | 73.7 ± 0.12 | 1.64 | −1.14 | 73.4 ± 1.2 | 1.64 | −0.77 | |
740 | 741.3 ± 5.6 | 0.76 | 0.59 | 731.3 ± 1.97 | 2.70 | 1.57 |