Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry

Table 1 The optimized MS/MS transitions and energy parameters of analytes

Analyte	DP (V)	CE (V)	CXP (V)	Precursor (amu)	Product (amu)
Astragaloside IV	117	81	17	806.9 [M + Na]⁺	627.1 [M–C₆H₁₁O₅–OH + Na]⁺
cycloastragenol	85	20	12	491.3 [M + H]⁺	143.0 [M–C₂₂H₃₆O₃ + H]⁺
Calycosin-7-O-β-d-glucoside	104	24	8	446.9 [M + H]⁺	284.2 [M–C₆H₁₁O₅ + H]⁺
Calycosin	117	33	17	285.0 [M + H]⁺	269.9 [M–CH₃ + H]⁺
Ononin	111	28	15	431.0 [M + H]⁺	268.2 [M–C₆H₁₁O₅ + H]⁺
Formononetin	80	50	17	269.0 [M + H]⁺	196.2 [M–OH–CO–OCH₃ + H]⁺
Scutellarin	100	29	8	463.0 [M + H]⁺	287.0 [M–C₆H₈O₆ + H]⁺