Table 1 Primers used for real-time RT-PCR
From: Nonsurgical periodontal treatment reduced aortic inflammation in ApoE−/− mice with periodontitis
Genes | Oligos | Primers 5′–3′ | Expected amplicon size (bp) | UniGene numbers |
|---|---|---|---|---|
GAPDH | F-primer | GTTGTCTCCTGCGACTTCA | 293 | Mm.304088 |
R-primer | GCCCCTCCTGTTATTATGG | |||
IL-6 | F-primer | TTCCATCCAGTTGCCTTCTTG | 134 | Mm.147620 |
R-primer | GGGAGTGGTATCCTCTGTGAAGTC | |||
IL-10 | F-primer | GCCCTTTGCTATGGTGTCCT | 106 | Mm.124092 |
F-primer | GCCCTTTGCTATGGTGTCCT | |||
VCAM-1 | F-primer | CTGGGGCAGGAAGTTAGATA | 270 | Mm.76649 |
R-primer | CAAGAAAAAGAAGGGGAGTCA | |||
CD68 | F-primer | GCTGTTCACCTTGACCTGCTC | 192 | Mm.15819 |
R-primer | GGTTGATTGTCGTCTGCGG | |||
MMP-9 | F-primer | CCGAGCTATCCACTCATCAAAC | 263 | Mm.4406 |
R-primer | CTGAACCATAACGCACAGACC |