Fig. 2From: Glutamate promotes nucleotide synthesis in the gut and improves availability of soybean meal feed in rainbow troutAlterations of gene expressions in distal intestine by oral Glu intake. Relative expression of genes related to nucleic acid synthesis in distal intestine. a cps2: carbamoyl-phosphate synthetase, b ppat: phosphoribosyl pyrophosphate amidotransferase, c pfas: phosphoribosylformylglycinamidine synthase. The tissues were collected before (initial), 30, 120, and 360 min after administration of 50, 500 mg/kg Glu p.o. The expression of the genes were estimated by quantitative RT-PCR and normalized by arbp. All values are expressed as the mean ± SEM (n = 6)Back to article page