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Table 1 Primer pair sequences used for real-time PCR amplifications

From: Influence of long (16L:8D) and short (8L:16D) photoperiods on blood metabolites and hepatic metabolism in Olive flounder, Paralichthys olivaceus

RT-PCR primer	5′–3′	Annealing temperature (°C)
HSL F	ACAGCAGTCACGGTTTGGT	60
HSL R	AGAGGAAGGCGTAGAGGGA	60
PEPCK F	GAACGGCTTCTTCGGTGT	60
PEPCK R	AAGCGGGAGTTGGGGTG	60
β-actin F	GGAAATCGTGCGTGACATTAAG	60
β-actin R	CCTCTGGACAACGGAACCTCT	60

HSL hormone-sensitive lipase; PEPCK phosphoenolpyruvate carboxykinase

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