Table 1 The relevant parameters of the fifteen reference genes
From: Identification and evaluation of reference genes in the Chinese white wax scale insect Ericerus pela
Gene symbol | Gene name | Primer sequences (5′–3′) | Amplicon length (bp) | Amplicon Tm (°C) | Regressionco efficient (R2) | Amplification efficiency (℅) |
|---|---|---|---|---|---|---|
ACT-1 | Actin | F: ATTGTAGGTCGTCTCGTGG R: AGTACTCCGTATGGATCG | 158 | 82 | 0.982 | 102.0 |
ACT-2 | Actin | F: GAATCGCTCTCCTCCGACTTTGAT R: GATCTACCATGTACCCAGGATTAC | 142 | 84.5 | 0.999 | 102.8 |
Tub | Tubulin | F: CTTCATGGGGTAGTAAACGCACTA R: GACAAATCGCGTGATGCAGCTGCAT | 102 | 80.0 | 0.994 | 91.6 |
βTub-1 | β-Tubulin | F: CTCATCCATACCCTCACCGGTGT R: ACCAAGAAGCTACTGCCGACG | 155 | 81.5 | 0.992 | 102.6 |
βTub-2 | β-Tubulin | F: AGTTCGGCACCTTCTGTGTAATGC R: GTTCCATCCCCAAAGGTCTCTGAT | 391 | 78.5 | 0.998 | 94.0 |
SdhA-1 | Succinate dehydrogenase, subunit A | F: CTAATGTTTCTACCAAGTCGGTAT R: GAATCGCGCGGTGCTCATGCTCGA | 107 | 76 | 0.997 | 95.5 |
SdhA-2 | Succinate dehydrogenase, subunit A | F: CTAATGCGGCATTAATACCACCT R: CACTGTTAAAGGATCCGATTGGT | 86 | 75 | 0.997 | 94.8 |
SdhA-3 | Succinate dehydrogenase, subunit A | F: GTATGCCACCCATATTGTAATGTAC R: CATGGAGCCAATCGATTGGGTGCCA | 148 | 79 | 0.997 | 95.6 |
RP II | RNA polymerase-II transcription | F: ATTTTCTTCGCCCTCTTGCA R: ATCGAGGCCATCTGCAAGG | 114 | 78.5 | 0.997 | 97.2 |
mRpL50-1 | Mitochondrial ribosomal protein L50 | F: CAGTATCAGGGTGGAATCTGTGATA R: GATGCCGGTAGTCCATGGCACGAT | 135 | 75.5 | 0.995 | 95.4 |
mRpL50-2 | Mitochondrial ribosomal protein L50 | F: AGTCCAACCATGGCCCTTATTAGCGA R: GTCATGTCAGCTTCTTCGGATGAGTG | 148 | 78.5 | 0.999 | 91.0 |
mRpL15 | Mitochondrial ribosomal protein L15 | F: GTACGCAGTATTACGCTTGGGCATG R: CATGTTTCATTGCGTTGACGATTCT | 135 | 77.5 | 0.998 | 92.7 |
UBQ-1 | Ployubiquitin | F: CTGAACGTGAATTTACCTCCTCTGTA R: GAAGCTGCTGAGGTCCTTCAATCAA | 256 | 78.5 | 0.998 | 92.2 |
UBQ-2 | Ployubiquitin | F: CATACGTGCTAGTCCAATGCTCAGC R: GATGCGATATGGATCAAGCACTGGT | 110 | 78 | 0.975 | 94.8 |
Myo | Myosin | F: ACGATCTCCCATCGAGATCAACA R: GATTACACCGAATTCACTCGCAT | 106 | 76.5 | 0.991 | 99.0 |