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Fig. 5 | SpringerPlus

Fig. 5

From: Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells

Fig. 5

Analysis of HDR mediated by TALEN or CRISPR/Cas9 using RFLP assay. a Hind II or Xho -digested, or co-digested genomic DNA isolated from cells derived from HEK293FTEGFP cells transfected with paired Cas9 or two TALEN pairs with ssODN1-BM and ssODN2-BM. NC: genomic DNA from untransfected HEK293FTEGFP cells was used as negative control. US: genomic DNA from unsorted HEK293FTEGFP cells transfect with indicated constructs. NG: genomic DNA from sorted EGFP negative cells. EBFP: genomic DNA from sorted EBFP positive cells from extended cultured EGFP negative populations. Red and green arrow head indicate the expected size of HindIII-digested and XhoI-digested product respectively. b Detection of targeted genomic deletions in differently sorted populations from HEK293FTEGFP cells transfected with indicated constructs. Symbols as shown in (a). c HindIII-digested genomic DNA isolated from cells derived from HEK293FTEGFP cells transfected with paired Cas9 or two TALEN pairs with EBFP-BM plasmid donor. Symbols as those shown in (a)

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