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Table 1 Enzymatic properties of PtrACS1 and PtrACS2 overexpressed in E. coli

From: Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa

  PtrACS1 PtrACS2
K m (mM) 0.25 ± 0.02 0.72 ± 0.03
V max (μM min−1 mg−1) 698.85 ± 32.05 245.96 ± 33.52
k cat (min−1) 166.77 143.45
k cat/K m (μM−1 min−1) 667.08 199.24
Optimal pH 7.5 8.0
Optimal temperature (°C) 35 35