Fig. 5
From: Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa
Expression and purification of recombinant PtrACS1 and PtrACS2. a Marker: Shanghai biochemical low molecular weight protein MARKER standard; lanes 1 and 2: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS1 not induced by IPTG; lane 3: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS1 induced by IPTG for 3Â h; lane 4: 250Â mM imidazole-eluted sample. b Marker: Shanghai biochemical low molecular weight protein MARKER standard; lane 1: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS2 not induced by IPTG; lane 2: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS2 induced by IPTG for 3Â h; lane 3: the flow-through sample; lane 4: 250Â mM imidazole-eluted sample