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Fig. 5 | SpringerPlus

Fig. 5

From: Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa

Fig. 5

Expression and purification of recombinant PtrACS1 and PtrACS2. a Marker: Shanghai biochemical low molecular weight protein MARKER standard; lanes 1 and 2: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS1 not induced by IPTG; lane 3: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS1 induced by IPTG for 3 h; lane 4: 250 mM imidazole-eluted sample. b Marker: Shanghai biochemical low molecular weight protein MARKER standard; lane 1: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS2 not induced by IPTG; lane 2: total protein from BL21 (DE3) cells harboring pET-30a(+)-ACS2 induced by IPTG for 3 h; lane 3: the flow-through sample; lane 4: 250 mM imidazole-eluted sample

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