Table 2 Percent inhibition, IC50 values of various cell lines treated with different concentrations of CoCl2·6H2O (5–100 mg/L) by using best fit regression model
From: Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
Concentration of CoCl2·6H2O (mg/L) (x) | ||||||||
|---|---|---|---|---|---|---|---|---|
Cell line | Inhibition (%) (y) | Regression equation | IC50 | |||||
5 | 10 | 25 | 50 | 75 | 100 | |||
293 T | 7.27 | 16.97 | 27.73 | 47.26 | 62.67 | 69.72 | y = 20.92 ln(x) − 31.03 R = 0.977*** | 48.1 |
IMR-32 | 31.24 | 66.60 | 77.22 | 78.10 | 79.01 | 79.22 | y = 13.59 ln(x) + 23.33 R = 0.849*** | 7.12 |
PC-3 | 19.80 | 31.58 | 49.70 | 67.98 | 80.36 | 82.08 | y = 21.86 ln(x) − 17.49 R = 0.995*** | 21.91 |
A549 | 0.817 | 5.48 | 46.92 | 73.61 | 80.02 | 80.79 | y = 30.61 ln(x) − 53.93 R = 0.980*** | 29.81 |
Two-way ANOVA summary table | ||||||
|---|---|---|---|---|---|---|
Source of variation | df | SS | MSS | F-ratio | Tukey’s HSD0.05 | 4.79 |
Treatment (cell line) | 3 | 8632.36 | 2877.45 | 470.06*** | ||
Dose (CoCl2·6H2O) | 5 | 39,715.85 | 7943.17 | 1297.59*** | ||
Treatment × dose (interaction) | 15 | 5669.54 | 377.97 | 61.75*** | ||
Error | 48 | 293.83 | 6.12 | |||
Total | 71 | 54,311.57 | ||||