Table 3 LOD, LODcopies and Tm value of the 19 real-time PCR assays
Detection element | Sensitivity LOD (%) | LODcopies | Tm value (°C) | DNA samples |
|---|---|---|---|---|
SPS | 0.01 | 5.53 | 85–86 | KMD |
Zein | 0.1 | 4.20 | 85.5 | Non GM maize |
Lectin | 0.1 | 10.39 | 83.6 | MON89788 |
HMG I/Y | 0.1 | 10.68 | 88.4 | Non GM rape |
pCaMV35s | 0.01 | 5.53 | 84.2–84.5 | KMD |
FMV35S | 0.1 | 7.56 | 74.8–75.5 | MON89034 |
T-35S | 0.01 | 5.53 | 75.7 | Topas19/2 |
T-E9 | 0.1 | 10.39 | 76.6–77.3 | MON89788 |
g7 | 0.01 | 19.45 | 76.6–76.9 | MS1 |
tNOS | 0.01 | 5.53 | 77.9–78.2 | TT51 |
Cry1Ab | 0.01 | 5.53 | 85.2–85.4 | KMD |
Cry2Ab | 0.1 | 7.56 | 88.3–88.6 | MON89034 |
NptII | 0.01 | 5.53 | 87.1–87.9 | KMD |
PAT | 0.1 | 14.27 | 84.5 | T45 |
BAR | 0.1 | 41.03 | 80.6–81 | RF3 |
18S | 0.01 | 4.20 | 85.1–85.8 | Non GM maize |
HPT | 0.01 | 5.53 | 88.5 | KMD |
Barnase | 0.1 | 19.45 | 85.6–86 | MS1 |
Barstar | 0.1 | 41.03 | 85.3–85.6 | RF3 |