Table 1 List of forward (F), reverse (R) primers used in this study
Assays | Primer sequences | Targeted sequences | Fragment size (bp) | References |
|---|---|---|---|---|
SPS | F-5′-ATCTGTTTACTCGTCAAGTGTCATCTC-3′ R-5′-GCCATGGATTACATATGGCAAGA-3′ | U33175, Oryza sativa sucrose phosphate synthase gene | 287 | Bulletin No. 1861-1-2012 of the Ministry of Agriculture of the People’s Republic of China (2012) |
Zein | F-5′-TGAACCCATGCATGCAGT-3′ R-5′-GGCAAGACCATTGGTGA-3′ | X07535, Maize delta zein structural10 gene | 173 | Cao et al. (2012) |
Lectin | F-5′-GCCCTCTACTCCACCCCCATCC-3′ R-5′-GCCCATCTGCAAGCCTTTTTGTG-3′ | K00821, Soybean lectin (Le1) gene | 118 | Bulletin No. 1485-6-2010 of the Ministry of Agriculture of the People’s Republic of China (2010) |
HMG I/Y | F-5′-TCCTTCCGTTTCCTCGCC-3′ R-5′-TTCCACGCCCTCTCCGCT-3′ | AF127919, Brassica napus high mobility group protein I/Y (HMGa) gene | 206 | Bulletin No. 869-4-2007 of the Ministry of Agriculture of the People’s Republic of China (2007) |
pCaMV35s | F-5′-CCATCATTGCGATAAAGGAAA-3′ R-5′-TCATCCCTTACGTCAGTGGAG-3′ | AJ783419, pCaMV35s element | 165 | Cao et al. (2012) |
FMV35S | F-5′-AAGACATCCACCGAAGACTTA-3′ R-5′-AGGACAGCTCTTTTCCACGTT-3′ | HB427176, FMV35S element | 210 | Pan et al. (2003) |
T-35S | F-5′-GTTTCGCTCATGTGTTGAGC-3′ R-5′-GGGGATCTGGATTTTAGTACTG-3′ | KF206153.1, T-35S element | 121 | Bulletin No. 1782-3-2012 of the Ministry of Agriculture of the People’s Republic of China (2012) |
T-E9 | F-5‘-GCCACGATTTGACACATTTTTACTC-3′ R-5′-CTGTGAAATGGAAATGGATGGAG-3′ | KT388099.1, T-E9 element | 166 | Tachezy et al. (2002) |
g7 | F-5′-AAGGCAATTTGTAGATGTTAATTCCC-3′ R-5′-ACATAATATCGCACTCAGTCTTTCATC-3′ | NC_017854.1, g7 element | 197 | This study |
tNOS | F-5′-ATCGTTCAAACATTTGGCA-3′ R-5′-ATTGCGGGACTCTAATCATA-3′ | AY255709, tNOS element | 165 | Cao et al. (2012) |
Cry1Ab | F-5′-GAAGGTTTGAGCAATCTCTAC-3′ R-5′-CGATCAGCCTAGTAAGGTCGT-3′ | AY326434, Cry1Ab gene | 301 | |
Cry2Ab | F-5′-CAGCGGCGCCAACCTCTACG-3′ R-5′-TGAACGGCGATGCACCAATGTC-3′ | NC_017203.1, Cry2Ab gene | 260 | Randhawa et al. (2010) |
NptII | F-5′-ACCTGTCCGGTGCCCTGAATGAACTGC-3′ R-5′-GCCATGATGGATACTTTCTCGGCAGGAGC-3′ | KF963132.1, NptII gene | 196 | Liu et al. (2010) |
PAT | F-5′-CGCGGTTTGTGATATCGTTAAC-3′ R-5′-TCTTGCAACCTCTCTAGATCATCAA-3′ | JX434028.1, PAT gene | 108 | Pan et al. (2003) |
bar | F-5′-ACAAGCACGGTCAACTTCC-3′ R-5′-ACTCGGCCGTCCAGTCGTA-3′ | KJ668650.1, bar gene | 175 | Pan et al. (2003) |
18S | F-5′-CCTGAGAAACGGCTACCAT-3′ R-5′-CGTGTCAGGATTGGGTAAT-3′ | KR048749.1, 18S ribosomal RNA gene | 137 | Pan et al. (2012) |
HPT | F-5′-GAAGTGCTTGACATTGGGGAGT-3′ R-5′-AGATGTTGGCGACCTCGTATT-3′ | AF234296, HPT gene | 472 | Bulletin No. 1782-2-2012 of the Ministry of Agriculture of the People’s Republic of China (2012) |
barnase | F-5′-CTGGGTGGCATCAAAAGGGAACC-3′ R-5′-TCCGGTCTGAATTTCTGAAGCCTG-3′ | M14442.1, barnase gene | 202 | Delano et al. (2003) |
barstar | F-5′-TCAGAAGTATCAGCGACCTCCACC-3′ R-5′-AAGTATGATGGTGATGTCGCAGCC-3′ | AY283058.1, barstar gene | 236 | Randhawa et al. (2010) |