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Table 5 ANOVA table of PmMuGST mRNA expression levels in the hepatopancreas and muscle and PmMuGST protein levels in the hepatopancreas of P. monodon after AFB1 exposure at 14, 28, 42 and 56 d (time, T) at different AFB1 concentrations (A)

From: Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Source

df

Sum of squares

Mean square

F

Pr > F

PmMuGST mRNA levels in the hepatopancreas (PmMuGST/EF1A)

 Corrected Model

23

24.710

1.074

28.467

0.000

 A

5

13.350

2.670

70.751

0.000

 T

3

1.550

0.517

13.695

0.000

 A × T

15

9.809

0.654

17.327

0.000

 Error

48

1.811

0.038

  

 Total

72

75.711

   

PmMuGST mRNA levels in the muscle (PmMuGST/EF1A)

 Corrected Model

23

107.151

4.659

39.906

0.000

 A

5

19.343

3.869

33.138

0.000

 T

3

30.156

10.052

86.104

0.000

 A × T

15

57.652

3.843

32.923

0.000

 Error

48

5.604

0.117

  

 Total

72

202.642

   

PmMuGST protein levels in the hepatopancreas (PmMuGST/GAPDH)

 Corrected Model

23

715.587

31.112

719.779

0.000

 A

5

98.396

19.679

455.274

0.000

 T

3

379.652

126.551

2927.720

0.000

 A × T

15

237.538

15.836

366.359

0.000

 Error

48

2.057

0.043

  

 Total

72

1235.345

  Â