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Table 5 ANOVA table of PmMuGST mRNA expression levels in the hepatopancreas and muscle and PmMuGST protein levels in the hepatopancreas of P. monodon after AFB1 exposure at 14, 28, 42 and 56 d (time, T) at different AFB1 concentrations (A)

From: Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Source df Sum of squares Mean square F Pr > F
PmMuGST mRNA levels in the hepatopancreas (PmMuGST/EF1A)
 Corrected Model 23 24.710 1.074 28.467 0.000
 A 5 13.350 2.670 70.751 0.000
 T 3 1.550 0.517 13.695 0.000
 A × T 15 9.809 0.654 17.327 0.000
 Error 48 1.811 0.038   
 Total 72 75.711    
PmMuGST mRNA levels in the muscle (PmMuGST/EF1A)
 Corrected Model 23 107.151 4.659 39.906 0.000
 A 5 19.343 3.869 33.138 0.000
 T 3 30.156 10.052 86.104 0.000
 A × T 15 57.652 3.843 32.923 0.000
 Error 48 5.604 0.117   
 Total 72 202.642    
PmMuGST protein levels in the hepatopancreas (PmMuGST/GAPDH)
 Corrected Model 23 715.587 31.112 719.779 0.000
 A 5 98.396 19.679 455.274 0.000
 T 3 379.652 126.551 2927.720 0.000
 A × T 15 237.538 15.836 366.359 0.000
 Error 48 2.057 0.043   
 Total 72 1235.345