Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

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Table 2 Oligonucleotide primers used in this study

Primers	Sequences (5′–3′)	Sequence information
MuGST-F1	CCTACGAGATCTTCGACCAGCACCT	Degenerate primers
MuGST-R1	CYYCTYCAYTTCRTATYTCTTCCTCT	Degenerate primers
MuGST-F2	CAGGCTTTCCAGAAGAGGTTTG	Nested degenerate primers
MuGST-R2	GATCGTAAACTGAGCGTACTTGTTGC	Nested degenerate primers
PmMuGST-F1	CAGGAAGTACATGGCGTCCCCGGATTTC	3′ RACE PCR
PmMuGST-F2	GTAGATGGCTTGGTTTATGAAGAGGAAGA	3′ RACE-nested PCR
PmMuGST-R1	TTCTTCCTCTTCATAAACCAAGCCATCTAC	5′ RACE PCR
PmMuGST-R2	CCTGATGAAATCCGGGGACGCCATGTAC	5′ RACE-nested PCR
EF1A-F	AGTATGCTCCTTTTGGACGTTTTGC	Real-time PCR
EF1A-R	CCTTTTCTGCGGCCTTGGTAGTC	Real-time PCR
PmMuGST-F	ACGGGCACTGAGTACGAGGAGAAG	Real-time PCR
PmMuGST-R	GGCAGATTTGGGAAAGCGAGG	Real-time PCR
MuGST-EF	CG GGATCCATGGTGCCTGTCCTGGG	Recombinant expression
MuGST-ER	CCC AAGCTTTCATTTTCCCTCAGCGATC	Recombinant expression

F and R stand for forward primers and reverse ones, respectively. MuGST-EF and MuGST-ER containing flanking non-complementary sequences (bold type) and the restriction sites (underlined). Y = C or T, R = A or G