Primers | Sequences (5′–3′) | Sequence information |
---|---|---|
MuGST-F1 | CCTACGAGATCTTCGACCAGCACCT | Degenerate primers |
MuGST-R1 | CYYCTYCAYTTCRTATYTCTTCCTCT | Degenerate primers |
MuGST-F2 | CAGGCTTTCCAGAAGAGGTTTG | Nested degenerate primers |
MuGST-R2 | GATCGTAAACTGAGCGTACTTGTTGC | Nested degenerate primers |
PmMuGST-F1 | CAGGAAGTACATGGCGTCCCCGGATTTC | 3′ RACE PCR |
PmMuGST-F2 | GTAGATGGCTTGGTTTATGAAGAGGAAGA | 3′ RACE-nested PCR |
PmMuGST-R1 | TTCTTCCTCTTCATAAACCAAGCCATCTAC | 5′ RACE PCR |
PmMuGST-R2 | CCTGATGAAATCCGGGGACGCCATGTAC | 5′ RACE-nested PCR |
EF1A-F | AGTATGCTCCTTTTGGACGTTTTGC | Real-time PCR |
EF1A-R | CCTTTTCTGCGGCCTTGGTAGTC | Real-time PCR |
PmMuGST-F | ACGGGCACTGAGTACGAGGAGAAG | Real-time PCR |
PmMuGST-R | GGCAGATTTGGGAAAGCGAGG | Real-time PCR |
MuGST-EF | CG GGATCCATGGTGCCTGTCCTGGG | Recombinant expression |
MuGST-ER | CCC AAGCTTTCATTTTCCCTCAGCGATC | Recombinant expression |