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Table 1 Ingredients and nutrient composition of the basal diet (g/100 g dry matter)

From: Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Ingredient

%

White fish meala

31

Soybean mealb

16.3

Peanut branb

17

Wheat flourb

19

Beer yeastb

5

Squid mealb

3

Soybean lecithinc

2

Fish oild

1

Soybean oila

1

Choline chloride (50 %)

0.6

Monocalcium phosphate

1

Ascorbic phosphate estere

0.1

Vitamin premixf

1

Mineral premixg

1

Sodium alginate

1

Proximate composition of each dieth (g/kg)

      

Groupi

D0

D1

D2

D3

D4

D5

Aflatoxin B1j (μg/kg)

0

50

100

500

1000

2500

Crude protein

47.31

47.26

47.35

47.61

47.44

47.56

Lipid

8.93

7.79

8.41

8.27

8.01

8.65

Ash

11.46

11.43

11.66

11.64

11.7

11.83

Moisture

9.65

9.80

9.75

9.71

9.75

9.61

  1. aImported from N.E.L.T.O. Australia Pty Ltd
  2. bZhuhai Shihai Feed Corporation Ltd, Zhuhai, China
  3. cKemin Industries (Zhuhai) Ltd., Zhuhai, China
  4. dImported from New Zealand (Bakels Edible Oils Ltd, Mt Macnganui)
  5. eGuangzhou Chengyi Company Ltd., Guangzhou, China
  6. fVitamin premix (g/kg): h-Carotene, 3 M.I.U.; Cholecalciferol, 0.6 M.I.U.; Thiamin, 3.6; Riboflavin, 7.2; Pyridoxine, 6.6; Cyanocobalamine, 0.02; a–Tocopherol, 16.5; Menadione, 2.4; Niacin, 14.4; Pantothenic acid, 4; Biotin, 0.02; Folic acid, 1.2; Inositol, 30; Ascorbic acid, 100; cellulose was used as a carrier
  7. gMineral premix (g/kg): P, 120; Ca, 120; Mg, 15; Fe, 1.5; Zn, 4.2; Cu, 2.1; K, 75; Co, 0.11; Mn, 1.6; Se, 0.01; Mo, 0.005; Al, 0.025; I, 0.4; cellulose was used as a carrier
  8. hMeasured values
  9. iGroup: D0, 0 μg/kg AFB1; D1, 50 μg/kg AFB1; D2, 100 μg/kg AFB1; D3, 500 μg/kg AFB1; D4, 1000 μg/kg AFB1; D5, 2500 μg/kg AFB1
  10. jAflatoxin B1 was purchased from Sigma (St. Louis, MO, USA)
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