Fig. 7

PmMuGST protein in the hepatopancreas of P. monodon at different time points after AFB1 exposure. PmMuGST protein was determined using western blot analysis with a PmMuGST antibody at 14, 28, 42 and 56 days post-exposure displayed in a–d, respectively. Equal amounts of proteins (30 μg) were subject to western blot analysis. GAPDH protein was used as an internal control. The amounts of GAPDH were also assessed to monitor the equal loadings of protein extracts. The optical density of the bands was determined directly on the film using IPP6.0 Analysis software. The relative intensity of objective protein was calculated based on the optical density ratio of PmMuGST and GAPDH bands. The different lowercase superscripts (a–f) represent significant differences in pairwise comparisons with control group (P < 0.05)