Fig. 5

Expression and purification of the recombination PmMuGST fusion protein. Equal amounts of proteins (30 μg) were subject to SDS-PAGE and western blotting analysis. a Protein samples were separated by SDS-PAGE and stained with Coomassie Brilliant Blue. Lane M, protein standard; lane 1, crude extract of BL 21 (DE3) without plasmid; lane 2, crude extract of the transformed BL21 (DE3) with recombined pET28a (+) plasmid induced with IPTG; lane 3, purified PmMuGST fusion protein. b Protein samples were analyzed by immunoblotting with anti-PmMuGST antibody. Lane M, protein standard; lane 1, crude extract of the transformed BL 21 (DE3) with recombined pET28a (+) plasmid induced with IPTG; lane 2, purified PmMuGST fusion protein