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Table 2 Effect of metal ions and organic reagents on the activity of purified PgluE8

From: NaCl-, protease-tolerant and cold-active endoglucanase from Paenibacillus sp. YD236 isolated from the feces of Bos frontalis

Reagent	Relative activity (%)^a
Reagent	10 mM	1 mM
None	100.0 ± 0.9	100.0 ± 0.1
β-Mercaptoethanol	176.6 ± 0.9	115.0 ± 0.5
KCl	128.7 ± 0.5	98.7 ± 0.3
NaCl	125.4 ± 0.6	98.2 ± 0.2
MgSO₄	125.0 ± 0.3	99.7 ± 0.5
CoCl₂	108.8 ± 1.3	102.8 ± 0.2
CuSO₄	104.5 ± 1.1	95.2 ± 0.2
CaCl₂	100.8 ± 1.1	97.3 ± 1.4
EDTA	98.5 ± 0.4	87.4 ± 0.9
ZnSO₄	84.4 ± 0.7	73.5 ± 0.7
NiSO₄	84.1 ± 1.1	88.9 ± 0.1
FeSO₄	77.8 ± 0.5	87.3 ± 0.3
MnSO₄	74.2 ± 0.8	95.6 ± 0.1
FeCl₃	72.8 ± 0.6	95.0 ± 0.3
PbAc	59.3 ± 0.7	78.6 ± 0.4
SDS	29.7 ± 0.9	11.2 ± 0.6
HgCl₂	10.8 ± 0.3	12.6 ± 0.3
AgNO₃	9.4 ± 0.3	10.2 ± 1.0

^aValues represent the mean ± SD (n = 3) relative to the untreated control samples

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