Table 1 Primers used to amplify the oriC of M. hyorhinis to construct pMD18-O/MHRgfp plasmid
From: GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis
Fragment | Enzymes | Oligonucleotides sequence (5′–3′) | Product (bp) |
|---|---|---|---|
oriC-MHR | EcoR1 | Forward: CCGGTGAATTCTACCTTTTGCTCTTCTTGCTGCTA | 1935 |
XhoI | Reverse: CGAACTCGAGTAGGAGGATTTCGTCTTATAGG |