Fig. 3
From: GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis
Detection of GFP fluorescence. M. hyorhinis was transformed with pMD18-O/MHRgfp, plated in KM2-Agar medium containing 0.01 µg/ml tetracycline hydrochloride and cultured for 7 days. Control mycoplasmas were transformed with a pMD18-T empty plasmid. a Expression of GFP gene upon transformation with pMD18-O/MHRgfp plasmid was observed under the fluorescence microscope. b The green fluorescence protein was not observed in the control mycoplasmas transformed with pMD18-T empty plasmid. Scale bar 100 µM