Fig. 2

pGL3 Basic plasmid and constructs on agarose gel. I ARMS products; the ARMS–PCR reactions were performed in two microtubes and the products (1624 bp) were run on the agarose gel (2 %) electrophoresis (Lanes A, B and Ladder). II Constructs containing three polymorphic sites were digested using restriction enzymes (REs) and were run on the agarose gel (3.5 %) electrophoresis (A amplified product, 568 bp, B digestion with SspI, 555 bp, C digestion with SspI and HindIII, 540 bp). III pGL3 Basic plasmid was digested using restriction enzymes (SmaI and HindIII) and was run on the agarose gel (2 %) electrophoresis (A intact plasmid, B digested plasmid with REs). IV The REs-digested constructs (539 bp) were inserted and ligated into pGL3 Basic plasmid and were compared on the agarose gel (2 %) (A construct-inserted plasmid, B intact plasmid)