Fig. 5

SNP detection from direct sequencing of PCR amplification and PCR plasmid of colonies from endosperm of callus and mother plants by primers of t6 (T. chinensis 13-alpha-hydroxylase gene, GenBank accession No. AY959321). Sixteen SNPs were detective by aligning with CLUSTAL OMEGA. The HD-25 is sequence of diploid PCR amplification, and seven sequences are diploid PCR amplification of plasmid of colonies, including of HD_t6_3_RV-M_A08_1209074553J, HD_t6_4_R V-M_B08_1209074554J, HD_t6_5_RV-M_C08_1209074555J, HD_t6_27_RV-M_B09_1209074577J, HD_t6_18_RV-M_F08_1209074568J, HD_t6_10_RV-M_D08_1209074560J, HD_t6_23_R V-M_H08_1209074573J. Five sequences are haploid PCR amplification of plasmid of colonies, including of HH_ 11_RV-M_D07_1209076658J, HH15_RV-M_E07_1209076662J, HH12_RV-M_G 07_1209076664J, HH_t6_12_RV-M_E07_1209 074591J, HH_t6_24_RV-M_G07_1209074603J