Fig. 4

SNP detection from direct sequencing of amplification PCR and PCR plasmid of colonies from endosperm of callus and mother plants by primers of LE2 (T. cuspidata var. nana LEAFY protein gene, GenBank accession No.: HQ245861). Three SNPs were detective by aligning with CLUSTAL OMEGA. The HD-12 is sequence of diploid PCR amplification, and two sequences are diploid PCR amplification of plasmid of colonies, including of HD_8_RV-M_D01_12090643475J and HD_17_RV-M_A02_12090643484J. The HH-2, HH-3 and HH-5 are the sequences of haploid PCR amplifications