Skip to main content
SpringerPlus

Table 2 Conditions for RT-qPCR

From: Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

Reversal transcription

Cycles:1

Temperature (°C)

Time (min:s)

50

10:00

Activation

Cycles : 1

Temperature (°C)

Time (min:s)

95

5:00

PCR

Cycles : 40

Temperature (°C)

Time (min:s)

95

00:10

60

00:30

Melting curve

Cycles : 1

Temperature (°C)

Time (min:s)

95

00:15

50

00:15

95

NA

  1. NA not applies
Back to article page