Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

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Table 1 Characteristics and concentration of primers used in this work

Primer name	Primer sequence	Length (pb)	Tm (°C)	ΔG of homodimers at 3′ (Kcal/mol)	ΔG of heterodimers at 3′ (Kcal/mol)	Conc. (nM)
DENV-For	TTGAGTAAACYRTGCTGCCTGTAGCTC	27	58.4–62.3	−6.34	−6.34	80
DENV-Rev	GAGACAGCAGGATCTCTGGTCTYTC	25	58.6–59.8	−6.28	−6.34	130
WNV-For	GGTGGATTTGGTTCTCGAAGGCG	23	60.4	−3.61	−3.61	60
WNV-Rev	AGGGTCAGCACGTTTGTCATTGTG	24	60	−1.95	−3.61	120
RICK-For	TATGCTTGCGGCTGTCGGTTCTC	23	61.7	−3.14	−3.61	1000
RICK-Rev	TTGCGGTAAGTTCGTAGTCTGCTTCTT	27	59.9	−3.61	−3.61	1000
LEP-For	AGCAGCCGCGGTAATACGTATGG	23	61.3	−3.14	−3.55	1000
LEP-Rev	TTTAGGGCGTGGATTACTGGGG	22	59	−0.96	−3.55	1000

Tm melting temperature, Conc. concentration used in this study, ΔG Gibbs energy