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Fig. 3 | SpringerPlus

Fig. 3

From: Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy

Fig. 3

Linearity ranges of the assays. Standard curves of the amplification plots are shown for the assays of Dengue virus (a), west Nile virus (b), Rickettsia (c), and Leptospira (d). Serial dilutions of 106–100 molecules of each positive control were used as a template for RT-qPCR reactions. The reactions were carried out in duplicate and Cycle threshold (Ct), efficiency, and slope were determined according to the Fix Points algorithm included in the Lightycler 480 II® software

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