Fig. 2

Blood samples were obtained from patients presenting fever during up to 5 days and were left at room temperature (RT) for 15 min for clotting. Samples were then transported from the hospital to the research center in a refrigerated container. Samples were centrifuged at 2000 rpm for 10 min in a cold centrifuge and recovered from the tubes inside a vertical laminar flow cabinet Class II Type A2 in BSL2 facilities. Lysis reagent of MagNA Pure LC total nucleic acid isolation kit was added to each sample and incubated for 15 min inside the laminar flow cabinet. 300 μL of inactivated serum were transferred into 32 well-plates for Roche MagNA Pure LC instrument. Total nucleic acids extractions were stored at −20 °C until RT-qPCR were performed