Table 1 Primers used in this study
Primer | Sequence (5′–3′) | Application | Annealing temperature (°C) |
|---|---|---|---|
ToF | CTTGGGTATGCCGTCTAT | Amplification of Tollip cDNA | 59 |
ToR | GTGCGAATCACCTCCTTA | Amplification of Tollip cDNA | 59 |
GSP3′-1 | CAGCCTGTGGTCCTGATG | 3′RACE specific out primer | 55 |
GSP3′-2 | CCTGCTGTTACATCACAGA | 3′RACE specific inner primer | 55 |
GSP5′-1 | GGTTCCCTCTCGCAGGCTCTCT | 5′RACE specific out primer | 55 |
GSP5′-2 | ACGCCCTCTCATCGAAGATTTC | 5′RACE specific inner primer | 55 |
U3′-1 | TACCGTCGTTCCACTAGTGATTT | 3′RACE universal out primer | 55 |
U3′-2 | CGCGGATCCTCCACTAGTGATTTCACTATAGG | 3′RACE universal inner primer | 55 |
U5′-1 | CATGGCTACATGCTGACAGCCTA | 5′RACE universal out primer | 55 |
U5′-2 | CGCGGATCCACAGCCTACTGATGATCAGTCGATG | 5′RACE universal inner primer | 55 |
S11F | CCGTGGGTGACATCGTTACA | Real-time PCR for S11 | 60 |
S11R | TCAGGACATTGAACCTCACTGTCT | Real-time PCR for S11 | 60 |
RT-TF | CCGTCTCAGCATCACCGTCGTA | Real-time PCR for Tollip | 60 |
RT-TR | TCGCTCCGTTGTGGGCAGTAG | Real-time PCR for Tollip | 60 |
IL-1βF | GCTCGGCTTCATCTTGGAGAATGT | Real-time PCR for IL-1β | 60 |
IL-1βR | GCAAGGTGAGGCTGGTCTTATTGT | Real-time PCR for IL-1β | 60 |
IL-6F | TGAAGACAGTGATGGAGCAGCAGA | Real-time PCR for IL-6 | 60 |
IL-6R | CCTCACAGCAATGTGGCGAACA | Real-time PCR for IL-6 | 60 |