Keratinolytic activities of alkaliphilic Bacillus sp. MBRL 575 from a novel habitat, limestone deposit site in Manipur, India

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Table 2 Effect of carbon and nitrogen supplements on keratinase, soluble peptides and amino acid production

	Keratinase (U/ml)	Soluble peptides (mg/g feather)	Amino acids (mg/g feather)
Carbon source (1 %, w/v)
Control^a	305 ± 12	120 ± 5.5	158 ± 4
Glucose	452 ± 6	136 ± 7	168 ± 14
Na Citrate	482 ± 16	150 ± 8.5	227 ± 18
Corn starch	418 ± 20	130 ± 4	176 ± 8
Lactose	280 ± 10	88 ± 6.2	102 ± 6.4
Sucrose	394 ± 14	129 ± 12	154 ± 2.4
Nitrogen source (0.5 %, w/v)
Peptone	350 ± 8	130 ± 6	176 ± 6
Yeast extract	338 ± 10	107 ± 16	130 ± 14
Soybean meal	536 ± 24	164 ± 10	263 ± 8
Beef extract	362 ± 6	125 ± 10.2	173 ± 12
KNO₃	209 ± 8	115 ± 8	54 ± 6
NH₄Cl	17 ± 3	52 ± 4	87 ± 4

^aControl represents CFM medium without any supplementary carbon or nitrogen source