Table 1 Effect of different factors on feather degradation by MBRL 575
Effect/treatment | Feather weight loss (%) | Keratinase activity (U/ml) | Soluble peptides (mg/g Feather) | Amino acids (mg/g feather) |
|---|---|---|---|---|
Temperature (°C) | ||||
 25 | 41 ± 3.6 | 80 ± 2 | 100 ± 2.5 | 109 ± 3.6 |
 28 | 79 ± 3.6 | 268 ± 4.04 | 111 ± 3.05 | 123 ± 3.6 |
 30 | 98 ± 0.4 | 305 ± 12 | 120 ± 5.5 | 158 ± 4 |
 35 | 48 ± 2 | 95 ± 3.05 | 102 ± 6.1 | 108 ± 2 |
Feather concentration (w/v %) | ||||
 0.5 | 100 ± 0.5 | 145 ± 7 | 161 ± 3 | 180 ± 6 |
 1.0 | 98 ± 0.4 | 305 ± 12 | 120 ± 5.5 | 158 ± 4 |
 2.0 | 85 ± 1 | 237 ± 11 | 110 ± 2.6 | 135 ± 2.5 |
 3.0 | 76 ± 2 | 139 ± 7.7 | 98 ± 2 | 116 ± 3.05 |
 5.0 | 61 ± 2.5 | 104 ± 6.02 | 80 ± 2.5 | 94.3 ± 4.04 |
 7.0 | 53 ± 2.5 | 77 ± 7.5 | 81 ± 5.56 | 94 ± 3 |
 10 | 45 ± 3 | 32 ± 4 | 81.3 ± 6.4 | 90 ± 2 |
Pre-treatment of feather | ||||
 Non-autoclaved | 46 ± 4 | 107 ± 8 | 58 ± 8.2 | 98 ± 2 |
 One cycle autoclaved | 98 ± 0.5 | 305 ± 12 | 120 ± 5.5 | 158 ± 4 |
 Two cycle autoclaved | 98 ± 2 | 310 ± 10 | 122 ± 4 | 162 ± 6 |
 Three cycle autoclaved | 99 ± 3 | 316 ± 7 | 126 ± 6.2 | 160 ± 8.2 |