Fig. 7From: Keratinolytic activities of alkaliphilic Bacillus sp. MBRL 575 from a novel habitat, limestone deposit site in Manipur, IndiaRelease of soluble peptides by cell free concentrated enzyme extract. 1 g feather was treated with 100 ml (glycine-NaOH buffer pH 10) concentrated enzyme (600 U/ml) and kept incubated at 40 °C. Aliquots are taken out at 1 h time interval and measured release of soluble peptide using Folin’s reagent. Each point represents the mean of three independent experimentsBack to article page