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Table 1 The strains and plasmids used in this study

From: Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineered Escherichia coli

Strains or plasmids Descriptions References
E. coli strains
 DH5α F , endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupGΦ80dlacZΔM15, Δ(lacZYA-argF)U169, hsdR17(rK mK + ), λ Lab stock
 BW25113 F , Δ(araBAD)567, ΔlacZ4787(::rrnB 3 ), λ , rp h-1, Δ(rhaBAD)568, hsdR514 Baba et al. (2006)
 ΔgadA JW3485, gadA null mutant of BW25113 Baba et al. (2006)
 ΔgadB JW1488, gadB null mutant of BW25113 Baba et al. (2006)
 ΔgadT JW2637, gabT null mutant of BW25113 Baba et al. (2006)
 ΔgadAB gadA and gadB null mutant of BW25113 This study
Plasmids
 pCP20 Flp+, λcI857+, λ PR Rep(pSC101 ori)ts, Apr, Cmr Datsenko and Wanner (2000)
 pYB1S p15A ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pRB1S RSF1020 ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pAB1S colA ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pDB1S cloDF13 ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pSB1S pSC101 ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pUB1S colE1 ori, arabinose-inducible araBAD promoter, Strr Lab stock
 pYB-bgadB pRB1S with gadB from L. brevis BH2 This study
 pYB-pgadB pRB1S with gadB from L. plantarum ATCC 14917 This study
 pYB-lgadB pRB1S with gadB from L. lactis IL1403 This study
 pAB-lgadB pAB1S with gadB from L. lactis IL1403 This study
 pDB-lgadB pDB1S with gadB from L. lactis IL1403 This study
 pSB-lgadB pSB1S with gadB from L. lactis IL1403 This study
 pUB-lgadB pUB1S with gadB from L. lactis IL1403 This study
 pRB-lgadB pYB1S with gadB from L. lactis IL1403 This study