Table 1 The strains and plasmids used in this study
Strains or plasmids | Descriptions | References |
|---|---|---|
E. coli strains | ||
DH5α | F − , endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupGΦ80dlacZΔM15, Δ(lacZYA-argF)U169, hsdR17(rK − mK + ), λ − | Lab stock |
BW25113 | F −, Δ(araBAD)567, ΔlacZ4787(::rrnB 3 ), λ − , rp h-1, Δ(rhaBAD)568, hsdR514 | Baba et al. (2006) |
ΔgadA | JW3485, gadA null mutant of BW25113 | Baba et al. (2006) |
ΔgadB | JW1488, gadB null mutant of BW25113 | Baba et al. (2006) |
ΔgadT | JW2637, gabT null mutant of BW25113 | Baba et al. (2006) |
ΔgadAB | gadA and gadB null mutant of BW25113 | This study |
Plasmids | ||
pCP20 | Flp+, λcI857+, λ PR Rep(pSC101 ori)ts, Apr, Cmr | Datsenko and Wanner (2000) |
pYB1S | p15A ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pRB1S | RSF1020 ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pAB1S | colA ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pDB1S | cloDF13 ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pSB1S | pSC101 ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pUB1S | colE1 ori, arabinose-inducible araBAD promoter, Strr | Lab stock |
pYB-bgadB | pRB1S with gadB from L. brevis BH2 | This study |
pYB-pgadB | pRB1S with gadB from L. plantarum ATCC 14917 | This study |
pYB-lgadB | pRB1S with gadB from L. lactis IL1403 | This study |
pAB-lgadB | pAB1S with gadB from L. lactis IL1403 | This study |
pDB-lgadB | pDB1S with gadB from L. lactis IL1403 | This study |
pSB-lgadB | pSB1S with gadB from L. lactis IL1403 | This study |
pUB-lgadB | pUB1S with gadB from L. lactis IL1403 | This study |
pRB-lgadB | pYB1S with gadB from L. lactis IL1403 | This study |