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Fig. 1 | SpringerPlus

Fig. 1

From: Detection of the rs10250202 polymorphism in protection of telomeres 1 gene through introducing a new restriction enzyme site for PCR–RFLP assay

Fig. 1

Ethidium bromide stained 3 % agarose gel showing the results of digestion with BclI. The 154 bp POT1 product was digested with BclI, which does not cut the wild-type Allele A sequence but cleaves the mutant-type Allele C sequence to yield two restriction fragments of 128 and 26 bp (it’s rarely to be seen in electropherogram). Lane 1 homozygous wild-type genotype (POT1-AA); Lane 2 heterozygous genotype (POT1-AC); Lane 3 homozygous mutant-type genotype (POT1-CC). M: molecular weight marker of 100 bp

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