Table 2 Evaluation of the three DNA markers used in the present study
| Â | rbcL | psbA-trnH | ITS2 |
|---|---|---|---|
PCR success (%) | 100 | 100 | 100 |
Amplified product length (bp) | 729–757 | 364–470 | 491–561 |
Aligned sequence length (bp) | 551 | 430 | 226 |
No. of variable sites | 5 | 6 | 13 |
No. of Pi sites | 4 | 5 | 11 |
No. of singletons | 1 | 0 | 2 |
Inter specific distance mean | 0.001 | 0.002 | 0.026 |
Total no. of clusters (UPGMA) | 3 | 3 | 4 |
Identification efficiency (%) | 15 | 50 | 100 |