Production of a microcapsule agent of chromate-reducing Lysinibacillus fusiformis ZC1 and its application in remediation of chromate-spiked soil

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Table 2 Experimental design and results of the orthogonal experiment

Run	A (g/L)	B (g/L)	C (g/L)	D (g/L)	Cell density (10⁸ cfu/mL)	Spore density (10⁸ cfu/mL)
1	2	4	4	3	1.00^a	0.25^a
2	2	8	8	6	1.65	0.7
3	2	12	12	9	1.60	0.1
4	4	4	8	9	1.50	0.2
5	4	8	12	3	1.85	0.45
6	4	12	4	6	1.90	0.4
7	6	4	12	6	2.00	0.85
8	6	8	4	9	2.40	0.8
9	6	12	8	3	3.00	0.55
k1^b	1.42	1.50	1.77	1.95
k2	1.75	1.97	2.05	1.85
k3	2.47	2.17	1.82	1.83
r^c	1.05	0.67	0.28	0.24
Optimal level	3	3	2	1
K1^b	0.33	0.43	0.48	0.42
K2	0.35	0.65	0.48	0.65
K3	0.73	0.33	0.35	0.35
R^c	0.4	0.32	0.13	0.3
Optimal level	3	2	2	2

^aValues indicate means of three replicates
^bk ^A_i is the mean of ∑cell density at A_i, K ^A_i is the mean of ∑spore density at A_i
^cr^A = max(k ^A_i ) − min(k ^A_i ), R^A = max(K ^A_i ) − min(K ^A_i )