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Table 1 Central composite design with observed and predicted responses of PGL and PG production under SSF by Bacillus tequilensis SV11-UV37

From: Retting and degumming of natural fibers by pectinolytic enzymes produced from Bacillus tequilensis SV11-UV37 using solid state fermentation

Run

A: Pectin (%)

B: Inoculum size (v/w)

C: Moisture level (%)

PGL observed (U/gds) ± SD

PGL Predicted (U/gds)

PG observed (U/gds) ± SD

PG Predicted (U/gds)

1

2.00

40.00

60.00

1401.25 ± 11.5

1370.02

91.97 ± 1.6

90.12

2

0.32

40.00

60.00

1534.38 ± 17.0

1562.74

97.365 ± 2.5

96.95

3

1.00

20.00

40.00

900.875 ± 5.2

867.29

91.395 ± 1.3

89.07

4

3.00

60.00

40.00

1448.75 ± 7.75

1427.84

72.15 ± 1.6

70.01

5

2.00

40.00

60.00

1341.88 ± 5.0

1370.02

88.4 ± 0.9

90.12

6

1.00

60.00

40.00

1802.5 ± 18.5

1786.04

95.03 ± 1.4

96.34

7

2.00

6.36

60.00

1293.75 ± 6.0

1334.63

85.43 ± 1.6

87.36

8

2.00

40.00

93.64

1220 ± 5.75

1234.40

102.1 ± 1.7

101.56

9

3.68

40.00

60.00

1533.13 ± 8.0

1558.93

81.03 ± 1.7

82.74

10

2.00

73.64

60.00

1693.13 ± 18.0

1706.42

80.79 ± 1.5

80.15

11

2.00

40.00

26.36

785 ± 5.5

824.77

80.96 ± 1.4

82.79

12

3.00

20.00

40.00

940 ± 8.25

913.02

76.93 ± 1.5

76.26

13

2.00

40.00

60.00

1411.88 ± 11.0

1370.02

91.19 ± 1.0

90.12

14

2.00

40.00

60.00

1343.75 ± 19.5

1370.02

88.31 ± 1.2

90.12

15

3.00

60.00

80.00

1334.38 ± 4.0

1329.66

86.07 ± 1.2

87.48

16

2.00

40.00

60.00

1378.75 ± 19.0

1370.02

91.4 ± 1.9

90.12

17

1.00

60.00

80.00

1391.25 ± 11.5

1379.93

91.83 ± 0.6

91.58

18

3.00

20.00

80.00

1828.13 ± 20.0

1806.28

105.55 ± 2.0

103.33

19

1.00

20.00

80.00

1470 ± 25.0

1452.61

92.69 ± 3.0

93.91

20

2.00

40.00

60.00

1351.88 ± 10.0

1370.02

89.67 ± 1.0

90.12

  1. SD standard deviation