Skip to main content

Table 2 Purification of l-asparaginase from Bacillus subtilis KDPS1

From: Mitigation of acrylamide by l-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC

Steps for purification

Total protein (mg)

Total activity (U/ml)

Specific activity (U/mg)

Fold purity

% yield

Crude enzyme

5.85 ± 3.34

46.87 ± 7.12

8.01 ± 3.62

1.00

100

Ammonium sulphate precipitation

1.12 ± 2.64

41.10 ± 6.15

36.69 ± 7.44

4.58

87.61

Ion exchange chromatography

0.41 ± 1.12

39.79 ± 4.71

97.04 ± 12.28

12.11

84.89