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Table 1 Effects of different medium conditions in shoot and root proliferation on grapevine (V. vinifera L.) buds cv. ‘Flame seedless’

From: Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds

Medium

Buds producing shoots (%) (mean ± SD)

Time of shoot induction (weeks)

Number of shoots per bud (mean ± SD)

Time of root induction (weeks)

Percentage of shoots showing roots (mean ± SD)

Shooting medium

 SM1

67 ± 1.5a

2

8 ± 2a

–

–

 SM2

20 ± 5.6

2

3 ± 1

–

–

Rooting medium

 RM1

–

–

–

8

0

 RM2

–

–

–

8

0

 RM3

–

–

–

3

62a

 RM4

–

–

–

6

20

  1. SM1, full strength MS with 8 µM 6-benzyl amino purine; SM2, full strength MS with 1 mg L−1 6-benzyl amino purine and 0.1 mg L−1 naphthalene acetic acid; RM1, full strength MS with 2.5 µM indole-3-butyric acid; RM2, full strength MS without hormone; RM3, half strength MS with 2 mg L−1 IBA and 200 mg L−1 activated charcoal; RM4, half strength MS with 2 mg L−1 indole-3-butyric acid and 3 g L−1 activated charcoal. Data are the mean of 12 buds per treatment replicated three times
  2. aDifferences among the treatments in a column according to Tukey–Kramer test (P < 0.05)
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