Fig. 2
The percentage of grapevine buds cv. ‘Flame seedless’ without contamination exposed to different isothiocyanate disinfection treatments on Murashige and Skoog medium at 26 °C. Ctr: buds washed with sterile distilled water, T2-60 (second control): buds treated with NaOCl (1.3 %) + Triton® X-100 incubated by 60 min. Buds treated with isothiocyanate solutions, A0.5-6 h: 0.5 mM Allyl incubated by 6 h, A0.5-12 h: 0.5 mM Allyl incubated by 12 h, A1-6 h: 1 mM Allyl incubated by 6 h, A1-12 h: 1 mM Allyl incubated by 12 h, A2.5-6 h: 2.5 mM Allyl incubated by 6 h, A2.5-12 h: 2.5 mM Allyl incubated by 12 h, B0.5-6 h: 0.5 mM Benzyl incubated by 6 h, B0.5-12 h: 0.5 mM Benzyl incubated by 12 h, B1-6 h: 1 mM Benzyl incubated by 6 h, B1-12 h: 1 mM Benzyl incubated by 12 h, B2.5-6 h: 2.5 mM Benzyl incubated by 6 h, B2.5-12 h: 2.5 mM Benzyl incubated by 12 h, P0.5-6 h: 0.5 mM Phenyl incubated by 6 h, P0.5-12 h: 0.5 mM Phenyl incubated by 12 h, P1-6 h: 1 mM Phenyl incubated by 6 h, P1-12 h: 1 mM Phenyl incubated by 12 h, P2.5-6 h: 2.5 mM Phenyl incubated by 6 h, P2.5-12 h: 2.5 mM Phenyl incubated by 12 h, PE0.5-6 h: 0.5 mM Phenylethyl incubated by 6 h, PE0.5-12 h: 0.5 mM Phenylethyl incubated by 12 h, PE1-6 h: 1 mM Phenylethyl incubated by 6 h, PE1-12 h: 1 mM Phenylethyl incubated by 12 h, PE2.5-6 h: 2.5 mM Phenylethyl incubated by 6 h, PE2.5-12 h: 2.5 mM Phenylethyl incubated by 12 h, Mix0.5-6 h: 0.5 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 6 h, Mix0.5-12 h: 0.5 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 12 h, Mix1-6 h: 1 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 6 h, Mix1-12 h: 1 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 12 h, Mix2.5-6 h: 2.5 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 6 h, Mix2.5-12 h: 2.5 mM Allyl:Benzyl:Pheniyethyl:Phenyl (1:3.5:5.3:9.6) incubated by 12 h. All treatments were maintained at 28 °C. Data represent the mean of 12 buds per treatment replicated three times. Asterisk indicates differences among the treatments according to Tukey–Kramer test at P < 0.05