Fig. 4

The protein levels of TRPs. a, c Western blots analysis and quantitative of TRPA1, TRPV1, TRPV2 and TRPM8 at various times after ION-CCI. b, d Western blots analysis and quantitative of TRPA1, TRPV1, TRPV2 and TRPM8 at 7 days after BoNT-A or normal saline injection (21 days after operation) in 4 treatment groups. β-actin was used as an internal standard. Only the representative Western blots of them are illustrated in this figure. Data were mean ± SD (n = 6/group). TG indicates injection into the trigeminal ganglion; WP indicates injection into the facial whisker pad. *P < 0.05 versus control and ^#P < 0.05 versus CCI group