Synthetic peptide number/amino acid sequences | Mast cell degranulation | Mast cell degranulation by WRK-treated peptide | Amino acid sequences of the peptide were replaced acids glutamic by leucine | Mast cell degranulation by Glu → Leu peptide |
---|---|---|---|---|
Mast cell isolation (negative control) | 21.60 ± 6.74 | 21.60 ± 6.74 | – | 21.60 ± 6.74 |
2S albumin (positive control) | 65.13 ± 8.5 | 65.13 ± 8.5 | – | 65.13 ± 8.5 |
P1/VRDICKKEAERRDLS | 59.21 ± 6.51 | 29.76 ± 3.66 | VRLICKKLALRRDLS | 43.95 ± 1.48 |
P4/QAKELSAICRCESIHYL | 69.08 ± 3.63 | 42.35 ± 0.49 | QAKLLSAICRCESIHYL | 38.96 ± 1.86 |
P5/LEKQLEEGEVGS | 71.46 ± 1.41 | 19.72 ± 1.23 | LLKQLEEGLVGS | 25.65 ± 5.44 |
P6/VGSEDEARRRTKNII | 66.84 ± 9.76 | 22.05 ± 1.48 | VGSLDLARRRTKNII | 32.36 ± 0.50 |
P8/SEDMLAMPGIEN | 60.95 ± 9.50 | 30.79 ± 1.11 | SLLMLAMPGIEN | 33.17 ± 0.23 |
P3/PRQQVPRQCCNQAKEL | 35.08 ± 4.34 | 24.97 ± 5.17 | PRQQVPRQCCN | |
P2/SCENYITQRRGR | 24.20 ± 2.30 | – | ||
P7/DVCFGSICPR | 32.06 ± 3.30 | – |