Fig. 5From: Identification of IgE-binding peptide and critical amino acids of Jatropha curcas allergen involved in allergenic responseELISA for IgE anti-Jat c 1 antibodies. Sera from rats immunized with Jat c 1 were assayed for Jat c 1 (control) and allergens from R. communis. Blanks 1, 2 and 3 contained water, PBS and pre-immune serum, respectively. IgE anti-Jat c 1 recognized a mixture of Ric c 1 and Ric c 3. Mean values from 5 experiments ± SD are presented. Statistical analysis was performed using one-way ANOVA followed by Turkey’s test. *p < 0.001Back to article page