Fig. 4From: Identification of IgE-binding peptide and critical amino acids of Jatropha curcas allergen involved in allergenic responseMast-cell degranulation assay. +Control: mast cells sensitized by Jat c 1 antiserum and incubated with Jat c 1; −Control: mast cells incubated with DMEM; large chain, small chain: mast cells sensitized by Jat c 1 antiserum and incubated with the large or small chain, respectively, isolated from Jat c 1 under denaturation, reduction and alkylation conditions. Mean values from 5 experiments ± SD are presented. Statistical analysis was performed using one-way ANOVA followed by Turkey’s test. *p < 0.001Back to article page