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Fig. 2 | SpringerPlus

Fig. 2

From: Identification of IgE-binding peptide and critical amino acids of Jatropha curcas allergen involved in allergenic response

Fig. 2

Reversed-phase chromatographic isolation of polypeptide chains from Jat c 1. Jat c 1 was treated with guanidine chloride, DTT and vinyl pyridine and was fractionated on a C18 column. a Blank reaction. b Elution profile of the Jat c 1 polypeptide chains after denaturation. Peak S (small chain) and Peak L (large chain). Elution conditions: solvent A, 0.1 % TFA; solvent B, 80 % acetonitrile/0.1 % TFA. The elution profile was monitored at 220 nm, and the oblique line represents the acetonitrile gradient. Insets in b. Spectra of Jat c 1 small (S) and large (L) chains

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