Fig. 3

SDS-PAGE and Western blotting analyses of homomultimeric fusion enzymes. Crude protein extracts from E. coli M15[pREP4] LB-medium expressing cel5A or fusion genes were separated on SDS-PAGE and visualized by Coomassie Brilliant Blue staining. Degradation products of homomultimeric fusion enzymes were detected using Western blotting analyses with specific antibodies for terminal affinity tags. Full length proteins are boxed in white. Cells were grown in 500 mL and harvested 4 h after induction with 0.5 mM IPTG