Fig. 2

Wet weights, total protein amounts and total activities of homomultimeric fusion enzymes. a Heterologous E. coli strains were grown in different incubation volumes and produced cell masses were determined. b Cells were disrupted by sonication, and insoluble proteins were sedimented. Concentration of separated soluble proteins was measured. c Total protein activities of soluble crude protein extracts were determined with the DNS-assay. Incubation volumes are indicated by the following colour code: 50 mL black, 100 mL dark grey, 250 mL middle grey, 500 mL light grey. Error bars indicate standard deviations of two to six independent measurements. Raw data from all measurements are given in Additional file 2