Fig. 1

Schematic diagram illustrating fusion enzymes, cloning strategy, and enzymatic activity. a Schemes displaying homomultimeric fusion constructs. Black boxes indicate HIS-tags, dark grey boxes highlight Cel5A and STREP-tags are given in light grey. Molecular weights are indicated aside. b Restriction analyses of cel5A-encoding plasmids using LguI and Eco81I. c Qualitative plate activity assays with AZCL-HE cellulose used as substrate